Epithelial cells lining salivary gland ducts are early target cells of severe acute respiratory syndrome coronavirus infection in the upper respiratory tracts of rhesus macaques.
Identifieur interne : 002119 ( Main/Exploration ); précédent : 002118; suivant : 002120Epithelial cells lining salivary gland ducts are early target cells of severe acute respiratory syndrome coronavirus infection in the upper respiratory tracts of rhesus macaques.
Auteurs : Li Liu [République populaire de Chine] ; Qiang Wei ; Xavier Alvarez ; Haibo Wang ; Yanhua Du ; Hua Zhu ; Hong Jiang ; Jingying Zhou ; Pokman Lam ; Linqi Zhang ; Andrew Lackner ; Chuan Qin ; Zhiwei ChenSource :
- Journal of virology [ 1098-5514 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux, Cellules épithéliales (virologie), Charge virale, Conduits salivaires (virologie), Immunohistochimie, Macaca mulatta, Microscopie, Peptidyl-Dipeptidase A (analyse), Récepteurs viraux (analyse), Syndrome respiratoire aigu sévère (médecine vétérinaire), Syndrome respiratoire aigu sévère (virologie), Virus du SRAS (isolement et purification), Virus du SRAS (pathogénicité).
- MESH :
- analyse : Peptidyl-Dipeptidase A, Récepteurs viraux.
- isolement et purification : Virus du SRAS.
- médecine vétérinaire : Syndrome respiratoire aigu sévère.
- pathogénicité : Virus du SRAS.
- virologie : Cellules épithéliales, Conduits salivaires, Syndrome respiratoire aigu sévère.
- Animaux, Charge virale, Immunohistochimie, Macaca mulatta, Microscopie.
English descriptors
- KwdEn :
- Animals, Epithelial Cells (virology), Immunohistochemistry, Macaca mulatta, Microscopy, Peptidyl-Dipeptidase A (analysis), Receptors, Virus (analysis), SARS Virus (isolation & purification), SARS Virus (pathogenicity), Salivary Ducts (virology), Severe Acute Respiratory Syndrome (veterinary), Severe Acute Respiratory Syndrome (virology), Viral Load.
- MESH :
- chemical , analysis : Peptidyl-Dipeptidase A, Receptors, Virus.
- isolation & purification : SARS Virus.
- pathogenicity : SARS Virus.
- veterinary : Severe Acute Respiratory Syndrome.
- virology : Epithelial Cells, Salivary Ducts, Severe Acute Respiratory Syndrome.
- Animals, Immunohistochemistry, Macaca mulatta, Microscopy, Viral Load.
Abstract
The shedding of severe acute respiratory syndrome coronavirus (SARS-CoV) into saliva droplets plays a critical role in viral transmission. The source of high viral loads in saliva, however, remains elusive. Here we investigate the early target cells of infection in the entire array of respiratory tissues in Chinese macaques after intranasal inoculations with a single-cycle pseudotyped virus and a pathogenic SARS-CoV. We found that angiotensin-converting enzyme 2-positive (ACE2(+)) cells were widely distributed in the upper respiratory tract, and ACE2(+) epithelial cells lining salivary gland ducts were the early target cells productively infected. Our findings also have implications for SARS-CoV early diagnosis and prevention.
DOI: 10.1128/JVI.02292-10
PubMed: 21289121
Affiliations:
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Le document en format XML
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(virology)</term><term>Immunohistochemistry</term><term>Macaca mulatta</term><term>Microscopy</term><term>Peptidyl-Dipeptidase A (analysis)</term><term>Receptors, Virus (analysis)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (pathogenicity)</term><term>Salivary Ducts (virology)</term><term>Severe Acute Respiratory Syndrome (veterinary)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Viral Load</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules épithéliales (virologie)</term><term>Charge virale</term><term>Conduits salivaires (virologie)</term><term>Immunohistochimie</term><term>Macaca mulatta</term><term>Microscopie</term><term>Peptidyl-Dipeptidase A (analyse)</term><term>Récepteurs viraux (analyse)</term><term>Syndrome respiratoire aigu sévère (médecine vétérinaire)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Cellules épithéliales</term><term>Conduits salivaires</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Epithelial Cells</term><term>Salivary Ducts</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Immunohistochemistry</term><term>Macaca mulatta</term><term>Microscopy</term><term>Viral Load</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Charge virale</term><term>Immunohistochimie</term><term>Macaca mulatta</term><term>Microscopie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The shedding of severe acute respiratory syndrome coronavirus (SARS-CoV) into saliva droplets plays a critical role in viral transmission. The source of high viral loads in saliva, however, remains elusive. Here we investigate the early target cells of infection in the entire array of respiratory tissues in Chinese macaques after intranasal inoculations with a single-cycle pseudotyped virus and a pathogenic SARS-CoV. We found that angiotensin-converting enzyme 2-positive (ACE2(+)) cells were widely distributed in the upper respiratory tract, and ACE2(+) epithelial cells lining salivary gland ducts were the early target cells productively infected. Our findings also have implications for SARS-CoV early diagnosis and prevention.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Alvarez, Xavier" sort="Alvarez, Xavier" uniqKey="Alvarez X" first="Xavier" last="Alvarez">Xavier Alvarez</name><name sortKey="Chen, Zhiwei" sort="Chen, Zhiwei" uniqKey="Chen Z" first="Zhiwei" last="Chen">Zhiwei Chen</name><name sortKey="Du, Yanhua" sort="Du, Yanhua" uniqKey="Du Y" first="Yanhua" last="Du">Yanhua Du</name><name sortKey="Jiang, Hong" sort="Jiang, Hong" uniqKey="Jiang H" first="Hong" last="Jiang">Hong Jiang</name><name sortKey="Lackner, Andrew" sort="Lackner, Andrew" uniqKey="Lackner A" first="Andrew" last="Lackner">Andrew Lackner</name><name sortKey="Lam, Pokman" sort="Lam, Pokman" uniqKey="Lam P" first="Pokman" last="Lam">Pokman Lam</name><name sortKey="Qin, Chuan" sort="Qin, Chuan" uniqKey="Qin C" first="Chuan" last="Qin">Chuan Qin</name><name sortKey="Wang, Haibo" sort="Wang, Haibo" uniqKey="Wang H" first="Haibo" last="Wang">Haibo Wang</name><name sortKey="Wei, Qiang" sort="Wei, Qiang" uniqKey="Wei Q" first="Qiang" last="Wei">Qiang Wei</name><name sortKey="Zhang, Linqi" sort="Zhang, Linqi" uniqKey="Zhang L" first="Linqi" last="Zhang">Linqi Zhang</name><name sortKey="Zhou, Jingying" sort="Zhou, Jingying" uniqKey="Zhou J" first="Jingying" last="Zhou">Jingying Zhou</name><name sortKey="Zhu, Hua" sort="Zhu, Hua" uniqKey="Zhu H" first="Hua" last="Zhu">Hua Zhu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Li" sort="Liu, Li" uniqKey="Liu L" first="Li" last="Liu">Li Liu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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